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KMID : 0525720120170020086
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Journal of Chitin and Chitosan
2012 Volume.17 No. 2 p.86 ~ p.90
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Inhibitory Effect of the Mixture of Deep-sea water and Chitosan Oligosaccharide on Expressions of VEGF and VEGFRs in Human Breast Cancer Cells
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Kwon Yun-Suk
Shin Jin-Sun
Lee Kyu-Shik
Nam Kyung-Soo
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Abstract
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Vascular endothelial growth factor (VEGF) has been thought as a crucial regulator of angiogenesis for development, growth, proliferation, and metastasis of breast cancer. VEGF promotes angiogenesis by binding its receptor tyrosine kinases (RTKs), VEGFR-1 and -2. In this study, we evaluated the effect of deep-sea water (DSW) on expressions of chitosan oligosaccharide (COS-L)-mediated VEGF and its receptors induced by phorbol-12-myristate-13-acetate (TPA) in MDA-MB-231 breast cancer cells. We found that the mixtures of COS-L 1 mg/mL and DSW with various hardness have not shown cytotoxicity on MDAMB- 231 cells in MTT assay, and also VEGF expression induced by TPA was significantly decreased in a range of 0.05 to 3 mg/ mL of COS-L. DSW of more than hardness 400 enhanced the COS-L-mediated inhibition of VEGF expression. In the case of VEGFR-1 and R-2, DSW of more than hardness 200 and 400 significantly increased the COS-L-mediated inhibition of VEGFR expressions, respectively. These results indicate that DSW improves the inhibitory effect of COS-L on the expressions of VEGF and its receptors. Therefore using together DSW and COS-L is effective for prevention of breast cancer by down-regulating the expressions of VEGF and its receptors that leads to inhibiting angiogenesis.
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KEYWORD
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Deep-Sea Water, Chitosan oligosaccharide, Breast cancer, VEGF, VEGF receptors
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